| First Author | Nian H | Year | 2011 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 52 |
| Issue | 8 | Pages | 5920-7 |
| PubMed ID | 21296823 | Mgi Jnum | J:181442 |
| Mgi Id | MGI:5311315 | Doi | 10.1167/iovs.10-6758 |
| Citation | Nian H, et al. (2011) Activated gammadelta T cells promote the activation of uveitogenic T cells and exacerbate EAU development. Invest Ophthalmol Vis Sci 52(8):5920-7 |
| abstractText | PURPOSE: To determine how the activation of gammadelta T cells affects the generation of uveitogenic alphabeta T cells and the development of experimental autoimmune uveitis (EAU). METHODS: gammadelta T cells were isolated from B6 mice immunized with the uveitogenic peptide IRBP(1-20) and alphabeta T cells from immunized TCR-delta(-/-) mice. Resting gammadelta T cells were prepared by culture of separated gammadelta T cells in cytokine-free medium for 3 to 5 days, when they showed downregulation of CD69 expression. Activated gammadelta T cells were prepared by incubating resting gammadelta T cells with anti-gammadelta TCR (GL3) for 2 days. Responder alphabeta T cells were cocultured with immunizing antigen and antigen-presenting cells. The numbers of antigen-specific T cells expressing IL-17 or IFN-gamma were determined by intracellular staining followed by FACS analysis after stimulation, with or without the addition of purified gammadelta T cells. The cytokines in the culture medium were measured by ELISA. RESULTS: Highly enriched gammadelta T cells exert widely different effects on autoreactive alphabeta T cells in EAU, depending on the activation status of the gammadelta T cells. Whereas nonactivated gammadelta T cells had little effect on the activation of interphotoreceptor retinoid-binding protein-specific alphabeta T cells in vitro and in vivo, activated gammadelta T cells promoted the generation of uveitogenic T cells and exacerbated the development of EAU. CONCLUSIONS: The functional ability of gammadelta T cells is greatly influenced by their activation status. Activated gammadelta T cells exacerbate EAU through increased activation of uveitogenic T cells. |
