| First Author | Reyes NJ | Year | 2011 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 52 |
| Issue | 5 | Pages | 2211-6 |
| PubMed ID | 21212171 | Mgi Jnum | J:171544 |
| Mgi Id | MGI:4950339 | Doi | 10.1167/iovs.10-5959 |
| Citation | Reyes NJ, et al. (2011) {gamma}{delta} T Cells Are Required for Maximal Expression of Allergic Conjunctivitis. Invest Ophthalmol Vis Sci 52(5):2211-6 |
| abstractText | Purpose. To determine the function of gammadelta T cells in early- and late-phase responses in allergic conjunctivitis. Methods. Wild-type (WT) C57BL/6 and gammadelta T cell-deficient (TCR-delta(-/-)) mice were immunized intraperitoneally and challenged topically for 7 consecutive days with short ragweed pollen. Natural killer T (NKT) and gammadelta T cell-double-deficient mice were generated by treating TCR-delta(-/-) mice with anti-CD1d antibody. Allergic conjunctivitis was evaluated clinically, and the late-phase response was assessed by histopathology. Cytokine profiles were evaluated by ELISA. The afferent and efferent arms of allergic conjunctivitis were assessed by adoptive transfer of CD4(+) T cells from WT or TCR-delta(-/-) mice into naive TCR-delta(-/-) or WT mice. Results. TCR-delta(-/-) mice had decreased clinical manifestations of allergic conjunctivitis compared with WT mice. TCR-delta(-/-) mice had decreased eosinophilic infiltration compared with WT mice. TCR-delta(-/-) mice produced less Th2-associated cytokines interleukin (IL)-4, -5, and -13 compared with WT mice. Clinical manifestations of allergic conjunctivitis were lowest in NKT cell-depleted TCR-delta(-/-) mice. However, late-phase allergic conjunctivitis in NKT cell-depleted, TCR-delta(-/-) mice was the same as TCR-delta(-/-) mice. Adoptive transfer of CD4(+) T cells revealed that gammadelta T cells are needed for the afferent and efferent arms of allergic conjunctivitis. Conclusions. gammadelta T cells are needed for full expression of both the clinical manifestations and the late phase of allergic conjunctivitis. Thus, gammadelta T cells have an important impact in the expression of allergic conjunctivitis and are a potential therapeutic target in the management of allergic diseases of the ocular surface. |
