| Primary Identifier | MGI:3525361 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ccn6 |
| Transmission | Germline | Strain of Origin | 129/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A lacZ-neo was inserted into the Wisp3 locus, replacing exons3-5 and creating a null allele. The allele resulted in a fusion protein that has Beta-gal activity under the endogenous promoter. The Wisp3 protein is truncated immediately after the IGFBP domain, and lacks the vWF, TSP and C-terminal domains. RT-PCR of cartilage from mutants showed a lack of wild-type transcript. Beta-gal activity was confirmed in cells transfected with a vector expressing the Wisp3-lacZ fusion protein. |
