| First Author | Adams AE | Year | 2008 |
| Journal | Biochim Biophys Acta | Volume | 1777 |
| Issue | 2 | Pages | 115-7 |
| PubMed ID | 17996719 | Mgi Jnum | J:133296 |
| Mgi Id | MGI:3778234 | Doi | 10.1016/j.bbabio.2007.10.003 |
| Citation | Adams AE, et al. (2008) Images of mitochondrial UCP 1 in mouse thymocytes using confocal microscopy. Biochim Biophys Acta 1777(2):115-7 |
| abstractText | The aim of this study was to demonstrate the constitutive expression of mitochondrial uncoupling protein 1 (UCP 1) in pure thymocytes using laser scanning confocal microscopic imagery. To that end we probed thymocytes from UCP 1 knock-out and wild-type mice. Mitochondrial location in thymocytes was determined using Mitotracker Red and the nucleus was labelled using Hoescht stain. We demonstrate that all cells investigated were thymocytes as determined by a monoclonal antibody specific for the thymocyte surface marker Thy 1 (CD90) pre-coupled to a fluorescent labelled (Alexa 448, green). Using a primary peptide antibody specific to UCP 1, and secondary fluorescently labelled (Alexa 647, magenta) antibody, we were able to demonstrate that UCP 1 is associated with mitochondria in thymocytes from UCP 1 wild-type mice but not thymocytes from UCP1-knock-out mice. These are the first images demonstrating the presence of UCP 1 in thymocyte mitochondria, in situ, and the first to clearly demonstrate UCP 1 expression in cells other than brown adipocytes. We conclude that mouse thymocytes contain UCP 1 in their mitochondria. |
