Primary Identifier | MGI:6119532 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Gfra1 |
Transmission | Germline | Strain of Origin | C57BL/6NTac |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | The targeting vector using bacterial artificial chromosomes (BACs) from the C57BL/6J RPCIB-731 BAC library was constructed by inserting a CreERT2 cassette and F3 flanked puromycin resistant cassette into the second exon of the Gfra1 gene in frame. The vector was transfected into the TaconicArtemis C57BL/6N Tac ESC line to establish mouse line by homologous recombination. Flp-mediated recombination removed the F3-flanked puro cassette. |