| First Author | Fukuhara R | Year | 2002 |
| Journal | Gene | Volume | 296 |
| Issue | 1-2 | Pages | 99-109 |
| PubMed ID | 12383507 | Mgi Jnum | J:79582 |
| Mgi Id | MGI:2388520 | Doi | 10.1016/s0378-1119(02)00837-5 |
| Citation | Fukuhara R, et al. (2002) Structure, molecular evolution, and gene expression of primate superoxide dismutases. Gene 296(1-2):99 |
| abstractText | Mn- and Cu,Zn-superoxide dismutase (SOD) cDNAs of eight primate species, Pan troglodytes, Pongo pygmaeus, Hylobates lar, Macaca fuscata, Macaca fascicularis, Macaca mulatta, Cebus apella, and Callithrix jacchus, were cloned. The whole protein-coding sequences were covered, comparing 198 and 153 (or 154) amino acids, for Mn- and Cu,Zn-SODs, respectively. Residues forming metal ligands were completely conserved in the two primate SODs and nucleotide/amino acid substitutions were more frequent in Cu,Zn-SODs than in Mn-SODs. Molecular evolutionary analyses showed Mn-SOD to have evolved at a constant rate and its phylogenetic tree well reflected primate phylogeny. Cu,Zn-SOD was shown to have evolved differently between primate lineages. The significant high ratio of a non-synonymous/synonymous rate was found in the lineage leading to great apes and humans, showing that this lineage underwent positive Darwinian selection. Southern hybridization suggested that the genes for primate Mn- and Cu,Zn-SOD exist as single copies. Northern analysis in various Japanese monkey tissues showed Mn- and Cu,Zn-SOD expression to be high in the liver, kidneys, and adrenal glands. |
