| First Author | de la Luna S | Year | 1996 |
| Journal | J Cell Sci | Volume | 109 ( Pt 10) |
| Pages | 2443-52 | PubMed ID | 8923205 |
| Mgi Jnum | J:36571 | Mgi Id | MGI:83999 |
| Doi | 10.1242/jcs.109.10.2443 | Citation | de la Luna S, et al. (1996) Nuclear accumulation of the E2F heterodimer regulated by subunit composition and alternative splicing of a nuclear localization signal. J Cell Sci 109(Pt 10):2443-52 |
| abstractText | The cellular transcription factor E2F plays a critical role in integrating cell cycle progression with the transcription apparatus by virtue of a physical interaction and control by key regulators of the cell cycle, such as pRb, cyclins and cyclin-dependent kinases. Generic E2F DNA binding activity arises when a member of two families of proteins, E2F and DP, form heterodimeric complexes, an interaction which results in co-operative transcriptional and DNA binding activity. Here, we characterise a new and hitherto unexpected mechanism of control influencing the activity of E2F which is mediated at the level of intracellular location through a dependence on heterodimer formation for nuclear translocation. Nuclear accumulation is dramatically influenced by two distinct processes: alternative splicing of a nuclear localization signal and subunit composition of the E2F heterodimer. These data define a new level of control in the E2F transcription factor whereby interplay between subunits dictates the levels of nuclear DNA binding activity. |
