|  Help  |  About  |  Contact Us

Publication : Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal.

First Author  Skoda RC Year  1993
Journal  EMBO J Volume  12
Issue  7 Pages  2645-53
PubMed ID  8334987 Mgi Jnum  J:12827
Mgi Id  MGI:61045 Doi  10.1002/j.1460-2075.1993.tb05925.x
Citation  Skoda RC, et al. (1993) Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal. EMBO J 12(7):2645-53
abstractText  The murine myeloproliferative leukemia virus has previously been shown to contain a fragment of the coding region of the c-mpl gene, a member of the cytokine receptor superfamily. We have isolated cDNA and genomic clones encoding murine c-mpl and localized the c-mpl gene to mouse chromosome 4. Since some members of this superfamily function by transducing a proliferative signal and since the putative ligand of mpl is unknown, we have generated a chimeric receptor to test the functional potential of mpl. The chimera consists of the extracellular domain of the human interleukin-4 receptor and the cytoplasmic domain of mpl. A mouse hematopoietic cell line transfected with this construct proliferates in response to human interleukin-4, thereby demonstrating that the cytoplasmic domain of mpl contains all elements necessary to transmit a growth stimulatory signal. In addition, we show that 25-40% of mpl mRNA found in the spleen corresponds to a novel truncated and potentially soluble isoform of mpl and that both full-length and truncated forms of mpl protein can be immunoprecipitated from lysates of transfected COS cells. Interestingly, however, although the truncated form of the receptor possesses a functional signal sequence and lacks a transmembrane domain, it is not detected in the culture media of transfected cells.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

17 Bio Entities

Trail: Publication

0 Expression