Primary Identifier | MGI:7624257 | Allele Type | Endonuclease-mediated |
Attribute String | Reporter | Gene | Ngf |
Strain of Origin | C57BL/6Ka | Is Recombinase | false |
Is Wild Type | false |
molecularNote | sgRNAs (CAATAGCTGCCCGAGTGACAGGG and AGTGTTTGGAGTCGATGCCCCGG)were designed to insert a red fluorescent protein (mScarlet) sequence, and woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) followed by a polyadenylation signal (mScarlet-WPRE-pA) after an alternative ATG start codon in exon 4, replacing most of the coding sequence in exon 4 of the endogenous nerve growth factor (Ngf) gene. |