| Primary Identifier | MGI:2448217 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Zyx |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| description | The authors report the 129SvJ GS embryonic stem cell line (Genome Systems, Inc., St.Louis, MO) was used for gene targeting. |
| molecularNote | The gene was disrupted by replacement of a 4.5 kb region of the gene containing a 5' non-coding exon and the first 4 exons encoding amino acids 1-333. Absence of gene expression was confirmed by Western blot analysis of lung and spleen mRNA from homozygous mutant animals. Multiple antibodies directed against N-terminal, central, and C-terminal residues did not detect any protein product. |
