| Primary Identifier | MGI:3806110 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Aff1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was produced as part of a proof of principle for a targeted gene trap scheme. |
| molecularNote | An Engrailed-2 splice acceptor was fused to the encephalomyocarditis virus internal ribosomal entry site (IRES) and place upstream of a lacZ-neomycin fusion reporter that had been modified by the insertion of a Tn903 promoter between the lacZ and the neo. This was followed by an SV40 polyadenylation signal and a Hygro reporter cassette under the control of an SV40 promoter. This construct was flanked by loxP sites and targeted into intron 3. |
