Primary Identifier | MGI:5316387 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Pik3c3 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
Project Collection | EUCOMM |
description | Cell line EPD0103_4_A07 was used to make chimeric mice. Germline transmission was accomplished and subsequenct recombination established the allele. J:182628 |
molecularNote | An FRT-flanked promoterless cassette, which contains a splice acceptor, beta-geo and polyadenylation sequence, with a 3' loxP site was inserted upstream of exon 4. An additional loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the promoterless cassette and left exon 4 floxed. |