| Primary Identifier | MGI:5431094 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Grin1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0469_5_G09 and EPD0469_5_C11 were successfully used to make chimeric mice. Germline transmission was accomplished. J:155845 J:193625 |
| molecularNote | A targeting vector containing a human beta-actin promoter-driven selection cassette consisting of a FRT site, lacZ sequence, the first loxP site, human beta-actin promoter driving the neomycin resistance gene, SV40 polyA, FRT site and a second loxP site was inserted downstream of exon 6 of the targeted gene, and another loxP site was inserted upstream of exon 6. Flp-mediated recombination removed the lacZ and neo cassette and left exon 6 floxed. |
