Primary Identifier | MGI:5703070 | Allele Type | Targeted |
Attribute String | Reporter, Transposon concatemer | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae (J:159351). |
molecularNote | A construct consisting of a CAG promoter, a single copy of a hyperactive Sleeping Beauty responsive transposon-stop cassette and a DsRed cassette was inserted by RMCE using ES cells carrying Col1a1tm13(neo/hygro*)Jae. Hyperactive Sleeping Beauty mediated transposition of the transposon results in expression of the reporter. |