| Primary Identifier | MGI:6156370 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Gad2 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6J)F1 |
| Is Recombinase | true | Is Wild Type | false |
| description | The targeting vector was electroporated into F1 hybrid of 129S6 x C57BL/6J ES cells derived by the Janelia Transgenic Facility (www.janelia.org). |
| molecularNote | The targeting vector containing a 10,389 bp genomic DNA fragment spanning exon 16 of mouse Gad2 and a cassette of IRES-FlpO-loxP2272-ACE-Cre POII NeoR-loxp2272 inserted between stop codon TAA and 3' UTR was introduced into ES cells. Positive ES clones were expanded for generation of chimeric mice which have gone through self excised NeoR cassette. |
