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Publication : Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element.

First Author  Wehn AK Year  2009
Journal  Genesis Volume  47
Issue  5 Pages  309-13
PubMed ID  19298012 Mgi Jnum  J:148512
Mgi Id  MGI:3845457 Doi  10.1002/dvg.20503
Citation  Wehn AK, et al. (2009) Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element. Genesis 47(5):309-313
abstractText  To study paraxial mesoderm formation in the mouse, transgenic lines that can be used to either selectively delete or express genes of interest in the paraxial mesoderm are required. We have generated a transgenic mouse line that expresses Cre recombinase in the paraxial mesoderm (PAM) beginning at e7.5. A lacZ Cre recombinase reporter line showed that in addition to PAM and its derivatives, lateral plate and intermediate mesoderm derivatives were also exposed to Cre activity, while the node, notochord, and cardiac mesoderm were not. We further demonstrate that 70-75% of the fibroblasts generated from Dll1-msd Cre, ROSA26-rtTA embryos possess Cre recombinase activity. These mice can therefore be used in combination with tet-responsive transgenic lines to generate mesoderm-derived embryonic fibroblasts that inducibly express a gene of interest. genesis 47:309-313, 2009. (c) 2009 Wiley-Liss, Inc.
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