| First Author | Wüthrich M | Year | 2006 |
| Journal | J Immunol | Volume | 176 |
| Issue | 9 | Pages | 5538-47 |
| PubMed ID | 16622023 | Mgi Jnum | J:131650 |
| Mgi Id | MGI:3774100 | Doi | 10.4049/jimmunol.176.9.5538 |
| Citation | Wuthrich M, et al. (2006) Differential requirements of T cell subsets for CD40 costimulation in immunity to Blastomyces dermatitidis. J Immunol 176(9):5538-47 |
| abstractText | Cell-mediated immunity and production of type 1 cytokines are the main defenses against pathogenic fungi. Ligation of CD40 by CD40L on T cells is critical for the induction of these immune responses in vivo. We explored the role of CD40/CD40L interactions in vaccine immunity to Blastomyces dermatitidis by immunizing CD40(-/-) and CD40L(-/-) mice and analyzing their resistance to reinfection in a murine pulmonary model. In the absence of CD40 or CD40L, CD4(+) cells failed to get primed or produce type 1 cytokine and impaired the generation of CD8(+) T1 cells. The CD8(+) T cell defect was not due to regulatory T cells or impaired APC maturation or Ag presentation to T cells. If CD4(+) cells were first eliminated, vaccination of CD40(-/-) and CD40L(-/-) mice restored priming of CD8(+) cells, type 1 cytokine production, and resistance. Hence, CD4(+) and CD8(+) cells differ sharply in their requirement for CD40/CD40L interaction during the generation of antifungal immunity. Despite the plasticity of T cell subsets in vaccine immunity, in absence of CD40/CD40L interaction, CD4(+) cells may impede the priming of CD8(+) cells at the cost of host survival against a lethal infectious disease. |
