| First Author | Hankeova S | Year | 2021 |
| Journal | Elife | Volume | 10 |
| PubMed ID | 33635272 | Mgi Jnum | J:319433 |
| Mgi Id | MGI:6805934 | Doi | 10.7554/eLife.60916 |
| Citation | Hankeova S, et al. (2021) DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome. Elife 10:e60916 |
| abstractText | Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1(Ndr/Ndr) mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1(Ndr/Ndr) liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models. |
