| First Author | Ji H | Year | 2022 |
| Journal | Mol Cell Endocrinol | Volume | 540 |
| Pages | 111506 | PubMed ID | 34801668 |
| Mgi Jnum | J:323818 | Mgi Id | MGI:6857574 |
| Doi | 10.1016/j.mce.2021.111506 | Citation | Ji H, et al. (2022) Let7b-5p inhibits insulin secretion and decreases pancreatic beta-cell mass in mice. Mol Cell Endocrinol 540:111506 |
| abstractText | MicroRNAs are crucial regulators for the development, mass and function of pancreatic beta-cells. MiRNA dysregulation is associated with beta-cell dysfunction and development of diabetes. The members of let7 family are important players in regulating cellular growth and metabolism. In this study we investigated the functional role of let7b-5p in the mouse pancreatic beta-cells. We generated pancreatic beta-cell-specific let7b-5p transgenic mouse model and analyzed the glucose metabolic phenotype, beta-cells mass and insulin secretion in vivo. Luciferase reporter assay, immunofluorescence staining and western blot were carried out to study the target genes of let7b-5p in beta-cells. Let7b-5p overexpression impaired the insulin production and secretion of beta-cells and resulted impaired glucose tolerance in mice. The overexpressed let7b-5p inhibited pancreatic beta-cell proliferation and decreased the expression of cyclin D1 and cyclin D2. Our findings demonstrated that let7b-5p was critical in regulating the proliferation and insulin secretion of pancreatic beta-cells. |
