| Primary Identifier | MGI:5571706 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cldn3 |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a single loxP site upstream of exon 1 and a frt-flanked neomycin resistance cassette followed by another loxP site downstream of exon 1. Cre-mediated recombination removed the floxed neo cassette and exon 1. Western blot analysis confirmed the absence of protein in the testes. Analysis by RT-qPCR confirmed the absence of transcript in the testes, liver and lung. |
