| First Author | Iannello RC | Year | 1994 |
| Journal | Reprod Fertil Dev | Volume | 6 |
| Issue | 5 | Pages | 599-604 |
| PubMed ID | 7569039 | Mgi Jnum | J:22371 |
| Mgi Id | MGI:70245 | Doi | 10.1071/rd9940599 |
| Citation | Iannello RC, et al. (1994) Mouse testis Pdha-2 promoter upstream sequences confer tissue-and temporal-specific activity in transgenic mice. Reprod Fertil Dev 6(5):599-604 |
| abstractText | Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the E1 alpha subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3.0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3.0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences. |
