| First Author | Straubinger AF | Year | 1999 |
| Journal | Gene | Volume | 236 |
| Issue | 2 | Pages | 273-80 |
| PubMed ID | 10452947 | Mgi Jnum | J:57037 |
| Mgi Id | MGI:1343606 | Doi | 10.1016/s0378-1119(99)00274-7 |
| Citation | Straubinger AF, et al. (1999) Identification of two transcripts of canine, feline, and porcine interleukin-1 alpha. Gene 236(2):273-80 |
| abstractText | Reverse transcription-polymerase chain reaction (RT-PCR) with interleukin-1alpha (IL-1alpha)-specific primers using total RNA from lipopolysaccharide (LPS)-stimulated lung macrophages resulted in the amplification of two distinct cDNA fragments. Cloning and sequencing of the canine and feline fragments revealed that, except for the absence of a specific 174 nucleotide sequence, the short and the long transcripts were identical. The in-frame 174 nucleotide deletion corresponds to exon 5 of the human and murine IL-1alpha gene, which encodes the cleavage site for calpain, a protein necessary for the processing of the IL-1alpha precursor into mature IL-1alpha. The two transcripts were found in the dog, cat and pig; analysis by RT-PCR, Southern and Northern blot hybridization showed no expression of the shorter IL-1alpha mRNA in equine or bovine macrophages. Expression of the two canine IL-1alpha transcripts was also detected in synovial membranes and was coordinately up-regulated in response to Borrelia burgdorferi infection under both in-vitro and in-vivo conditions. |
