| First Author | Sakamoto H | Year | 2006 |
| Journal | Blood | Volume | 108 |
| Issue | 3 | Pages | 896-903 |
| PubMed ID | 16597594 | Mgi Jnum | J:139202 |
| Mgi Id | MGI:3807453 | Doi | 10.1182/blood-2005-09-3846 |
| Citation | Sakamoto H, et al. (2006) Proper levels of c-Myb are discretely defined at distinct steps of hematopoietic cell development. Blood 108(3):896-903 |
| abstractText | The definitive hematopoietic cell lineages have been proposed to originate from hemogenic endothelial cells during mouse embryogenesis. c-Myb is a transcription factor that is essential for the development of definitive hematopoiesis. To investigate the functional role of c-Myb in hematopoietic cell development from endothelial cells, we introduced a c-myb transgene expressed under the control of a tetracycline-regulated promoter into the c-myb(-/-) embryonic stem (ES) cell line, with the aim of inducing c-Myb expression at any stage and at any level. Induction of c-Myb expression after replating c-myb(-)(/)(-) endothelial cells rescued the generation and proliferation of definitive hematopoietic progenitor cells, suggesting that c-Myb expression in developing endothelial cells is not a prerequisite for their hematogenic potential. Overexpression of c-Myb, however, prevented the terminal differentiation of erythrocytes and megakaryocytes and completely abolished B-lymphocyte development. Our results indicate that c-Myb is a major factor that controls differentiation as well as proliferation of hematopoietic progenitor cells derived from hemogenic endothelial cells, and that appropriate levels of c-Myb protein are strictly defined at distinct differentiation steps of each hematopoietic cell lineage. |
