| First Author | Yamamoto A | Year | 2018 |
| Journal | J Vet Med Sci | Volume | 80 |
| Issue | 4 | Pages | 557-567 |
| PubMed ID | 29526868 | Mgi Jnum | J:313622 |
| Mgi Id | MGI:6791684 | Doi | 10.1292/jvms.18-0023 |
| Citation | Yamamoto A, et al. (2018) The mechanisms underlying the effects of AMH on Mullerian duct regression in male mice. J Vet Med Sci 80(4):557-567 |
| abstractText | Anti-Mullerian hormone (AMH) produced in the developing testis induces the regression of the Mullerian duct, which develops into the oviducts, uterus and upper vagina. In our true hermaphrodite mouse with an ovary on one side and a testis on the other (O/T), the oviduct and uterus are present only on the ovary side, and nothing derived from the Mullerian duct is present on the testis side. Here, we investigate the mechanism underlying the unilateral Mullerian duct regression and the mode of AMH signaling, by performing immunohistology, Western blotting, and organ culture analyses. The histological analysis revealed that during the start of the Mullerian duct regression, the duct in the O/T mice was clearly regressed on the AMH-positive testis side compared to the AMH-negative ovary side. The immunohistochemistry showed a diffuse immunoreaction of AMH in the interstitium surrounding the testis cord and boundary region between the testis and mesonephros, especially in the cranial portion. Western blotting revealed that the amount of AMH in the cranial half of the mesonephros was larger than that in the caudal half. AMH injected into the gonads in organ culture induced the regression of the Mullerian duct via the interstitium of the organ. These results suggest that AMH acts on the Mullerian duct in male mice by exuding into the interstitium surrounding the testis cord and infiltrating through the cranial region from the testis to the mesonephros. |
