| First Author | Albuck AL | Year | 2020 |
| Journal | Am J Physiol Heart Circ Physiol | PubMed ID | 33164581 |
| Mgi Jnum | J:299692 | Mgi Id | MGI:6501073 |
| Doi | 10.1152/ajpheart.00389.2020 | Citation | Albuck AL, et al. (2020) Peroxynitrite Decomposition Catalyst Enhances Respiratory Function in Isolated Brain Mitochondria. Am J Physiol Heart Circ Physiol |
| abstractText | Peroxynitrite (PN), generated from the reaction of nitric oxide (NO) and superoxide, is implicated in the pathogenesis of ischemic and neurodegenerative brain injuries. Mitochondria produce NO from mitochondrial NO synthases and superoxide by the electron transport chain. Our objective was to detect the generation of PN of mitochondrial origin and characterize its effects on mitochondrial respiratory function. METHODS: Freshly isolated brain non-synaptosomal mitochondria from C57Bl/6 (wild type, WT) and endothelial NO synthase knock out (eNOS-KO) mice were treated with exogenous PN (0.1, 1, 5 micromol/L) or a PN donor (SIN-1; 50 micromol/L), or PN scavenger (FeTMPyP; 2.5 micromol/L). Oxygen consumption rate (OCR) was measured using Agilent Seahorse XFe24 analyzer and mitochondrial respiratory parameters were calculated. Mitochondrial membrane potential, superoxide, and PN were determined from rhodamine 123, dihydroethidium, and DAX-J2 PON green fluorescence measurements, respectively. Mitochondrial protein nitrotyrosination was determined by western blots. RESULTS: Both exogenous PN and SIN-1 decreased respiratory function in WT isolated brain mitochondria. FeTMPyP enhanced state III and state IVo mitochondrial respiration in both WT and eNOS-KO mitochondria. FeTMPyP also elevated state IIIu respiration in eNOS-KO mitochondria. Unlike PN, neither SIN-1 nor FeTMPyP depolarized the mitochondria. Although mitochondrial protein nitrotyrosination was unaffected by SIN-1 or FeTMPyP, FeTMPyP reduced mitochondrial PN levels. Mitochondrial superoxide levels were increased by FeTMPyP but unaffected by PN or SIN-1. CONCLUSIONS: Thus, we present the evidence of functionally significant PN generation in isolated brain mitochondria. Mitochondrial PN activity was physiologically relevant in WT mice and pathologically significant under conditions with eNOS deficiency. |
