| Primary Identifier | MGI:5476700 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Gsk3a |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_gt1 cassette was inserted at position 24934747 of Chromosome 7 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 24935591. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. Western blot analysis confirmed normal protein expression levels in the kidney. |
