| First Author | Shebzukhov YV | Year | 2014 |
| Journal | Eur J Immunol | Volume | 44 |
| Issue | 1 | Pages | 251-64 |
| PubMed ID | 24009130 | Mgi Jnum | J:208215 |
| Mgi Id | MGI:5562491 | Doi | 10.1002/eji.201243297 |
| Citation | Shebzukhov YV, et al. (2014) Dynamic changes in chromatin conformation at the TNF transcription start site in T helper lymphocyte subsets. Eur J Immunol 44(1):251-64 |
| abstractText | Tumor necrosis factor (TNF) is one of the key primary response genes in the immune system that can be activated by a variety of stimuli. Previous analysis of chromatin accessibility to DNaseI demonstrated open chromatin conformation of the TNF proximal promoter in T cells. Here, using chromatin probing with restriction enzyme EcoNI and micrococcal nuclease we show that in contrast to the proximal promoter, the TNF transcription start site remains in a closed chromatin configuration in primary T helper (Th) cells, but acquires an open state after activation or polarization under Th1 and Th17 conditions. We further demonstrate that transcription factor c-Jun plays a pivotal role in the maintenance of open chromatin conformation at the transcription start site of the TNF gene. |
