| First Author | Mowat M | Year | 1983 |
| Journal | J Virol | Volume | 47 |
| Issue | 3 | Pages | 471-7 |
| PubMed ID | 6620461 | Mgi Jnum | J:16562 |
| Mgi Id | MGI:64631 | Doi | 10.1128/jvi.47.3.471-477.1983 |
| Citation | Mowat M, et al. (1983) Linkage of the Fv-2 gene to a newly reinserted ecotropic retrovirus in Fv-2 congenic mice. J Virol 47(3):471-7 |
| abstractText | Restriction enzyme and Southern gel analyses were used to determine the number and location of endogenous ecotropic retroviruses in the germ line of several mouse strains congenic at the Fv-2 gene locus. A new endogenous ecotropic provirus was observed in the germ line of B6.S (Fv-2ss) mice, in addition to the resident provirus found in its congenic partner C57BL/6 (Fv-2rr). This new provirus was similar in structure to the C57BL provirus. The SIM strain of mice, the donors of the Fv-2s allele in B6.S mice, does not contain ecotropic proviruses, suggesting that the new provirus in the B6.S mouse strain arose by germ-line reintegration during the construction of this strain. Mendelian segregation analysis indicated that this new provirus was linked to the Fv-2 gene locus on chromosome 9. In three other Fv-2s congenic mouse strains--B10.C (47N), B6.C (H-7b), and C57BL/6J Trfa, Bgsd--no additional ecotropic endogenous viruses were detected, suggesting that the reinsertion event that occurred during the construction of B6.S is not essential for the acquisition of the Fv-2s phenotype in the C57BL genetic background. Although numerous reports of germ-line reinsertions of ecotropic virus in high-virus mouse strains have been received, the present results provide definitive evidence that similar germ-line amplifications of endogenous ecotropic virus can occur in a low-virus mouse strain. |
