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Allele : E2f3<tm1.1Gev> E2F transcription factor 3; targeted mutation 1.1, Gerard Evan

Primary Identifier  MGI:6719199 Allele Type  Targeted
Attribute String  Inducible, No functional change Gene  E2f3
Transmission  Germline Strain of Origin  129
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  A vector containing 15 kb of the endogenous genomic sequence. The tetracycline response element (TRE) element was isolated from pTre2 (Clontech) and then cloned 500bp upstream of the translation start site along with a loxP flanked neomycin resistance cassette. No endogenous gene sequences were removed or altered at the insertion site, although silent mutations were incorporated into the first exons of E2f3a and E2f3b. Finally, a DTA cassette was added to this vector to allow negative selection in mouse embryonic stem cells. Cre-mediated recombination removed the conditional neomycin resistance cassette.
  • mutations:
  • Insertion
  • synonyms:
  • E2f3<TRE>,
  • E2f3<TRE>,
  • E2f3<tm1.1(TRE)Gev>,
  • E2f3<tm1.1(TRE)Gev>
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

3 Publication categories

Trail: Allele