| First Author | Hoeflich A | Year | 2002 |
| Journal | FEBS Lett | Volume | 523 |
| Issue | 1-3 | Pages | 63-7 |
| PubMed ID | 12123805 | Mgi Jnum | J:78152 |
| Mgi Id | MGI:2183627 | Doi | 10.1016/s0014-5793(02)02935-6 |
| Citation | Hoeflich A, et al. (2002) Mutation of the RGD sequence does not affect plasma membrane association and growth inhibitory effects of elevated IGFBP-2 in vivo. FEBS Lett 523(1-3):63-7 |
| abstractText | Using insulin-like growth factor-binding protein-2 (IGFBP-2) transgenic mice (D mice) as a model of elevated IGFBP-2 expression, which is often found in unphysiological conditions, we found association of IGFBP-2 to purified plasma membranes of many organs. To determine whether the RGD (Arg-Gly-Asp) motif of IGFBP-2 mediates cell surface binding in vivo, we mutated the RGD motif of IGFBP-2 into an RGE (Arg-Gly-Glu) sequence and produced transgenic mice (E mice) which express elevated amounts of mutated IGFBP-2. Our data demonstrate that in vivo IGFBP-2 cell surface association is not dependent on the RGD motif and that mutation of this sequence does not alter growth inhibitory effects of IGFBP-2. |
