| First Author | Brilliant MH | Year | 1991 |
| Journal | Science | Volume | 252 |
| Issue | 5005 | Pages | 566-9 |
| PubMed ID | 1673574 | Mgi Jnum | J:11138 |
| Mgi Id | MGI:59580 | Doi | 10.1126/science.1673574 |
| Citation | Brilliant MH, et al. (1991) Direct molecular identification of the mouse pink-eyed unstable mutation by genome scanning. Science 252(5005):566-9 |
| abstractText | DNA sequences associated with the mouse pink-eyed unstable mutation were identified in the absence of closely linked molecular markers and without prior knowledge of the encoded gene product. This was accomplished by genome scanning, a technique in which high-resolution Southern blots of genomic DNAs were hybridized to a dispersed and moderately repetitive DNA sequence. In this assay, pink-eyed unstable DNA was distinguished from the DNA of wild-type and revertant mice by enhanced hybridization to one of several hundred resolved fragments. The fragment showing enhanced hybridization in pink-eyed unstable DNA was cloned and found to lie within a DNA duplication that is located close to, or within, the pink-eyed dilution locus. The duplication associated with the mouse pink-eyed unstable mutation may mediate the high reversion frequency characteristic of this mutation. |
