| First Author | Scharf R | Year | 2016 |
| Journal | Sci Rep | Volume | 6 |
| Pages | 28381 | PubMed ID | 27334849 |
| Mgi Jnum | J:265497 | Mgi Id | MGI:6101840 |
| Doi | 10.1038/srep28381 | Citation | Scharf R, et al. (2016) Depth-specific optogenetic control in vivo with a scalable, high-density muLED neural probe. Sci Rep 6:28381 |
| abstractText | Controlling neural circuits is a powerful approach to uncover a causal link between neural activity and behaviour. Optogenetics has been widely adopted by the neuroscience community as it offers cell-type-specific perturbation with millisecond precision. However, these studies require light delivery in complex patterns with cellular-scale resolution, while covering a large volume of tissue at depth in vivo. Here we describe a novel high-density silicon-based microscale light-emitting diode (muLED) array, consisting of up to ninety-six 25 mum-diameter muLEDs emitting at a wavelength of 450 nm with a peak irradiance of 400 mW/mm(2). A width of 100 mum, tapering to a 1 mum point, and a 40 mum thickness help minimise tissue damage during insertion. Thermal properties permit a set of optogenetic operating regimes, with ~0.5 degrees C average temperature increase. We demonstrate depth-dependent activation of mouse neocortical neurons in vivo, offering an inexpensive novel tool for the precise manipulation of neural activity. |
