| First Author | Wen R | Year | 2004 |
| Journal | EMBO J | Volume | 23 |
| Issue | 20 | Pages | 4007-17 |
| PubMed ID | 15372077 | Mgi Jnum | J:93280 |
| Mgi Id | MGI:3056719 | Doi | 10.1038/sj.emboj.7600405 |
| Citation | Wen R, et al. (2004) An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion. EMBO J 23(20):4007-17 |
| abstractText | Phospholipase Cgamma1 (PLCgamma1) has been reported to be expressed predominantly in T cells and to play an important role in T-cell receptor signaling. Here we show that PLCgamma1 is expressed throughout B-cell development, with high expression in B-cell progenitors, and is involved in pre-B-cell receptor (pre-BCR) signaling. Reduced expression of PLCgamma1, in the absence of PLCgamma2 (PLCgamma1(+/-)PLCgamma2(-/-)), impedes early B-cell development at the pro-B- to pre-B-cell transition and impairs immunoglobulin heavy chain allelic exclusion, hallmarks of defective pre-BCR signaling. In contrast, early B-cell development is largely normal, whereas late B-cell maturation is impaired in the absence of PLCgamma2 alone (PLCgamma2(-/-)) and overexpression of PLCgamma1 in PLCgamma2(-/-) mice fails to restore BCR-mediated B-cell proliferation and maturation. These studies reveal an essential role of PLCgamma1, distinct from that of PLCgamma2, in B-cell development. |
