| First Author | Yannoutsos N | Year | 2004 |
| Journal | Nat Immunol | Volume | 5 |
| Issue | 4 | Pages | 443-50 |
| PubMed ID | 15021880 | Mgi Jnum | J:90487 |
| Mgi Id | MGI:3043960 | Doi | 10.1038/ni1053 |
| Citation | Yannoutsos N, et al. (2004) A cis element in the recombination activating gene locus regulates gene expression by counteracting a distant silencer. Nat Immunol 5(4):443-50 |
| abstractText | We have identified a silencer and an antisilencing element that interact at a distance of 85 kilobases to regulate expression of the recombination activating genes Rag1 and Rag2 in thymocytes. Transgenic experiments showed that Rag promoter-proximal cis elements directed tissue-specific expression and that a Runx-dependent intergenic silencer suppressed expression in developing T cells. Deletion of the antisilencing element from the genomic Rag locus unmasked the intergenic silencer and abrogated Rag expression in developing CD4(+)CD8(+) T cells. We speculate that the Rag antisilencing element belongs to a class of cis elements that might be useful for genome diversification by activating genes encoded by otherwise silent transposable elements. |
