| First Author | Zhang FX | Year | 1996 |
| Journal | Cell Mol Neurobiol | Volume | 16 |
| Issue | 4 | Pages | 479-87 |
| PubMed ID | 8879750 | Mgi Jnum | J:36370 |
| Mgi Id | MGI:83803 | Doi | 10.1007/BF02150228 |
| Citation | Zhang FX, et al. (1996) Expression of platelet-derived growth factor (PDGF) receptor alpha-subunit in mouse brain: comparison of Patch mutants and normal littermates. Cell Mol Neurobiol 16(4):479-87 |
| abstractText | 1. Platelet-derived growth factor (PDGF) plays an important role not only in mesenchyme-derived tissues, but also in the mammalian central nervous system. The Patch mutant (Ph/+) lacks one copy of the PDGFR-alpha gene, However, it is not clear whether there are differences in expression of PDGF receptor alpha-subunit (PDGFR-alpha) in brain tissue of the Patch heterozygous (Ph/+) mutants compared to wild-type C57B1 (+/+) mice. 2. The level of PDGRF-alpha mRNA expression is slightly lower in Patch mutant than in normal littermate. 3. Protein and total RNA isolated from mouse brain tissue and primary type 1 astrocyte cultures were studied with Western and Northern blotting techniques, There was no measurable difference in PDGFR-alpha protein expression between the Patch and wild- type mouse nervous system. Adjustment of transcriptional efficiency and messenger stability may contribute to this phenomenon, whose biological significance remains unclear. 4. Further, the expression of PDGRF-alpha protein and message in mouse brain tissues is developmentally regulated. Its level remains high during the embryonic period and declines below measurable levels in adult. |
