| First Author | Tiedeman AA | Year | 1991 |
| Journal | Gene | Volume | 97 |
| Issue | 2 | Pages | 289-93 |
| PubMed ID | 1671845 | Mgi Jnum | J:11016 |
| Mgi Id | MGI:59458 | Doi | 10.1016/0378-1119(91)90065-j |
| Citation | Tiedeman AA, et al. (1991) Isolation and sequence of a cDNA encoding mouse IMP dehydrogenase. Gene 97(2):289-93 |
| abstractText | Inosinic acid (IMP) dehydrogenase (IMPD) catalyzes the conversion of IMP to XMP as the first committed step in GMP biosynthesis de novo. We have isolated a cDNA containing the complete coding region of mouse IMPD by its ability to complement a bacterial mutant lacking IMPD activity. Two independent cDNA clones were isolated by complementation, of which the longest was 1.7 kb in length. Northern analyses, using the IMPD cDNA as a probe, indicated that mature IMPD mRNA was a single species approx. 2.0 kb in size. Mouse IMPD is almost identical to Chinese hamster and human IMPDs and is highly conserved between Escherichia coli and mouse, with a direct amino acid (aa) identity of 39%, which increases to 60% if conserved aa are considered. The leader region of our longest cDNA clone is G + C-rich and contains two tandem copies of a G + C-rich direct repeat. |
