| Primary Identifier | MGI:4951042 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Null/knockout, Reporter | Gene | Cryba1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Special note about the mutation generated in J:235696: qRT-PCR analysis confirmed the absence of mRNA transcripts in homozygous mutant lenses. Western blot analysis using a polyclonal anti-betaA3-crystallin antibody revealed that reactivity corresponding to a dimer (50 kDa) is completely absent in homozygous lenses and reduced to 50% in heterozygous lenses. However, a band of severely reduced intensity is still detectable in homozygous lenses at the 25 kDa position. Similar results are observed using a monoclonal antibody, suggesting that a small amount of protein is still produced in homozygous lenses. |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 77611369 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77612125. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
