| Primary Identifier | MGI:2149670 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Ephb2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Insertion of a LacZ-neomycin resistance cassette in-frame at codon 622. Western blot analysis using a polyclonal Ephb2 antibody did not detect protein in cell surface immunoprecipitates of primary brain cultures from homozygous mutant neonates (P4), however a polyclonal beta-galactosidase antibody did detect a novel 200 kDa protein in these cultures. The authors propose that the mutant allele produces a fusion receptor protein comprised of the extracellular, transmembrane, and juxtamembrane domains (amino acids 1-621) linked to beta-galactosidase, and that this mutated receptor lacks the entire tyrosine kinase catalytic and C-terminal domains (amino acids 622-707). |
