| First Author | Takagi T | Year | 1993 |
| Journal | Biochim Biophys Acta | Volume | 1178 |
| Issue | 2 | Pages | 194-200 |
| PubMed ID | 8347678 | Mgi Jnum | J:14184 |
| Mgi Id | MGI:62358 | Doi | 10.1016/0167-4889(93)90009-e |
| Citation | Takagi T, et al. (1993) Identification of the product of the murine ST2 gene. Biochim Biophys Acta 1178(2):194-200 |
| abstractText | The murine ST2 gene is expressed in growth-stimulated BALB/c-3T3 cells. This gene encodes a protein that is similar to the extracellular portions of the interleukin-1 receptors (types 1 and 2). In this study, we prepared a polyclonal antibody against the recombinant ST2 protein produced in Escherichia coli. This antibody detected recombinant ST2 protein in the culture fluid of COS7 cells transfected with a mammalian expression vector (pEF-BOS) carrying ST2 cDNA. Using this antibody, we could detect the ST2 protein in the culture fluid of growth-stimulated BALB/c-3T3 cells, and in the medium of continuously growing cells, but not in that of growth-arrested cells. ST2 proteins produced in COS7 cells and BALB/c-3T3 cells were N-glycosylated as predicted from nine putative N-glycosylation sites in its deduced amino-acid sequence. |
