| First Author | Gaucher D | Year | 2002 |
| Journal | Gene | Volume | 294 |
| Issue | 1-2 | Pages | 233-8 |
| PubMed ID | 12234685 | Mgi Jnum | J:79801 |
| Mgi Id | MGI:2388952 | Doi | 10.1016/s0378-1119(02)00795-3 |
| Citation | Gaucher D, et al. (2002) Molecular cloning and expression of gerbil granulocyte/macrophage colony-stimulating factor. Gene 294(1-2):233 |
| abstractText | Using a combination of cross species reverse transcriptase-polymerase chain reaction and 3' rapid amplification of cDNA ends techniques, we cloned the cDNA encoding gerbil granulocyte/macrophage colony-stimulating factor (GM-CSF). The open reading frame had 81% nucleotide identity with its mouse counterpart, while the mature protein had 80% homology with mature mouse GM-CSF. COS-7 cells transfected with gerbil GM-CSF cDNA secreted high levels of bioactive GM-CSF, as their supernatant stimulated gerbil bone-marrow cell proliferation and colony formation in semi-solid medium. |
