| First Author | Komai-Koma M | Year | 2009 |
| Journal | Eur J Immunol | Volume | 39 |
| Issue | 6 | Pages | 1564-72 |
| PubMed ID | 19405031 | Mgi Jnum | J:149561 |
| Mgi Id | MGI:3848675 | Doi | 10.1002/eji.200838866 |
| Citation | Komai-Koma M, et al. (2009) Direct recognition of LPS by human but not murine CD8+ T cells via TLR4 complex. Eur J Immunol 39(6):1564-72 |
| abstractText | LPS comprises a major PAMP and is a key target of the immune system during bacterial infection. While LPS can be recognised by innate immune cells via the TLR4 complex, it is unknown whether T lymphocytes, especially CD8(+) T cells are also capable of doing so. We report here that naive human CD8(+) T cells, after activation by TCR stimulation, express surface TLR4 and CD14. These activated CD8(+) T cells can then secrete high concentrations of IFN-gamma, granzyme and perforin in response to LPS. These effects can be specifically inhibited using siRNA for TLR4. Furthermore, LPS can synergize with IL-12 to polarize the CD8(+) T cells into cytotoxic T-cell 1 (Tc1) that produce IFN-gamma but not IL-4, with or without TCR activation. Moreover, CD8(+)CD45RO(+) memory T cells constitutively expressed TLR4 and markedly enhanced IFN-gamma production when stimulated with LPS. In contrast, activated murine CD8(+) T cells lack TLR4 and CD14 expression and fail to respond to LPS for proliferation and cytokine production. Thus, human but not murine CD8(+) T cells are able to directly recognise bacterial LPS via LPS receptor complex and TLR4 provides a novel signal for the activation of effector and memory human CD8(+) T cells. |
