| First Author | Wang Z | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 18 | Pages | 4057-62 |
| PubMed ID | 20732323 | Mgi Jnum | J:164387 |
| Mgi Id | MGI:4833746 | Doi | 10.1016/j.febslet.2010.08.028 |
| Citation | Wang Z, et al. (2010) GPR48-Induced keratinocyte proliferation occurs through HB-EGF mediated EGFR transactivation. FEBS Lett 584(18):4057-62 |
| abstractText | GPR48 can mediate keratinocyte proliferation and migration. Our investigations showed that AG1478, an inhibitor of EGFR tyrosine kinase, could block GPR48-mediated cellular processes. AG1478 treatment of Gpr48(+/+) cells also decreased phosphorylation of EGFR, ERK and STAT3. Subsequent screening using conditioned media immunodepleted of EGFR ligands identified HB-EGF as the ligand responsible for phosphorylation of EGFR, ERK and STAT3. HB-EGF was reduced in Gpr48(-/-) cell culture medium, but its addition restored the phosphorylation of EGFR, ERK, STAT3, as well as cell proliferation. Confirmation that GPR48 mediates EGFR signaling pathway through HB-EGF was subsequently performed using an inhibitor of HB-EGF. |
