| First Author | Esteva-Font C | Year | 2014 |
| Journal | FASEB J | Volume | 28 |
| Issue | 3 | Pages | 1446-53 |
| PubMed ID | 24334548 | Mgi Jnum | J:210686 |
| Mgi Id | MGI:5571664 | Doi | 10.1096/fj.13-245621 |
| Citation | Esteva-Font C, et al. (2014) Aquaporin-1 gene deletion reduces breast tumor growth and lung metastasis in tumor-producing MMTV-PyVT mice. FASEB J 28(3):1446-53 |
| abstractText | Aquaporin 1 (AQP1) is a plasma membrane water-transporting protein expressed strongly in tumor microvascular endothelia. We previously reported impaired angiogenesis in implanted tumors in AQP1-deficient mice and reduced migration of AQP1-deficient endothelial cells in vitro. Here, we investigated the consequences of AQP1 deficiency in mice that spontaneously develop well-differentiated, luminal-type breast adenomas with lung metastases [mouse mammary tumor virus-driven polyoma virus middle T oncogene (MMTV-PyVT)]. AQP1(+/+) MMTV-PyVT mice developed large breast tumors with total tumor mass 3.5 +/- 0.5 g and volume 265 +/- 36 mm(3) (SE, 11 mice) at age 98 d. Tumor mass (1.6+/-0.2 g) and volume (131+/-15 mm(3), 12 mice) were greatly reduced in AQP1(-/-) MMTV-PyVT mice (P<0.005). CD31 immunofluorescence showed abnormal microvascular anatomy in tumors of AQP1(-/-) MMTV-PyVT mice, with reduced vessel density. HIF-1alpha expression was increased in tumors in AQP1(-/-) MMTV-PyVT mice. The number of lung metastases (5+/-1/mouse) was much lower than in AQP1(+/+) MMTV-PyVT mice (31+/-8/mouse, P<0.005). These results implicate AQP1 as an important determinant of tumor angiogenesis and, hence, as a potential drug target for adjuvant therapy of solid tumors. |
