| First Author | Oh-hashi K | Year | 2011 |
| Journal | FEBS Lett | Volume | 585 |
| Issue | 15 | Pages | 2481-7 |
| PubMed ID | 21729698 | Mgi Jnum | J:176035 |
| Mgi Id | MGI:5288159 | Doi | 10.1016/j.febslet.2011.06.029 |
| Citation | Oh-hashi K, et al. (2011) Biosynthesis and secretion of mouse cysteine-rich with EGF-like domains 2. FEBS Lett 585(15):2481-7 |
| abstractText | In this study, we found that Cysteine-rich with EGF-like domains 2 (CRELD2), a novel endoplasmic reticulum stress-inducible protein, is not only localized in the ER-Golgi apparatus but also spontaneously secreted. Deletion of four C-terminal amino acids from mouse CRELD2 or addition of tag-peptides to its C-terminus dramatically enhanced CRELD2 secretion. Intra- and extra-cellular CRELD2 is differentially glycosylated and its spontaneous secretion was significantly prevented by overexpression of a dominant negative mutant Sar1 and treatment with brefeldin A. Overexpression of wild-type GRP78 remarkably enhanced the secretion of wild-type but not mutant CRELD2. Our results demonstrate both that CRELD2 is a novel secretory glycoprotein regulated by Sar1 and GRP78 and that the C-terminal of CRELD2 plays a crucial role in its secretion. |
