| First Author | Usui K | Year | 2012 |
| Journal | Mol Immunol | Volume | 50 |
| Issue | 3 | Pages | 172-6 |
| PubMed ID | 22189408 | Mgi Jnum | J:185097 |
| Mgi Id | MGI:5427332 | Doi | 10.1016/j.molimm.2011.11.010 |
| Citation | Usui K, et al. (2012) Isolation and characterization of naive follicular dendritic cells. Mol Immunol 50(3):172-6 |
| abstractText | Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naive mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for ~0.2% of the spleen cells of naive mice, were CD45(-), FDC-M2(+), and ICAM-1(+), and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation. |
