Primary Identifier | MGI:7546839 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector is designed with (from 5' to 3') a ubiquitously expressed CAG promoter, a loxP-flanked stop cassette, a Venus reporter sequence, 3F2A (three tandem repeats of foot-and-mouth disease 2A peptide sequence) that mediates ribosomal skipping, the Apex2 gene sequence with a nuclear localization signal, a WPRE sequence, and an FRT-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the neo cassette. |