| First Author | Braga M | Year | 2012 |
| Journal | Mol Cell Endocrinol | Volume | 350 |
| Issue | 1 | Pages | 39-52 |
| PubMed ID | 22138414 | Mgi Jnum | J:185676 |
| Mgi Id | MGI:5429648 | Doi | 10.1016/j.mce.2011.11.019 |
| Citation | Braga M, et al. (2012) Testosterone inhibits transforming growth factor-beta signaling during myogenic differentiation and proliferation of mouse satellite cells: potential role of follistatin in mediating testosterone action. Mol Cell Endocrinol 350(1):39-52 |
| abstractText | Testosterone (T) administration is associated with increased satellite cell number and skeletal muscle hypertrophy, although there is considerable heterogeneity in the response of different skeletal muscle groups to T in vivo. We investigated the effects of T on the growth and differentiation of satellite cells isolated from levator ani (LA) and gastrocnemius (gastroc) muscles. T up regulated follistatin (Fst) expression, but down regulated the mRNA and protein expression of a number of genes in the transforming growth factor-beta (TGF-beta)-signaling pathway. Inhibition of Fst expression by small interfering RNA (siRNA) inhibited myogenic differentiation and blocked the pro-myogenic effects of T. Treatment of satellite cells with T or Fst up regulated the expression of Pax7 and PCNA, and increased their proliferation. T and Fst blocked TGF-beta induced inhibition of growth and myogenic differentiation and down regulated TGF-beta-dependent transcriptome in both LA and gastroc cells. We conclude that T stimulation of satellite cell proliferation and myogenic differentiation are associated with up regulation of Fst and inhibition of TGF-beta-signaling. |
