Primary Identifier | MGI:7593593 | Allele Type | Targeted |
Attribute String | Humanized sequence | Gene | Gnptab |
Transmission | Germline | Strain of Origin | BALB/cJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to replace exon 19 with one specifying a change of glutamic acid to lysine at amino acid position 1179 (c.3535G>A (GAA-AAA)) , which is homologous to a human Glu1200Lys mutation correlated with stuttering. The targeting vector also contained an internal ribosome entry site (IRES) to allow initiation of translation of an inserted beta-galactoside gene (beta-gal) for subsequent gene expression studies in tissues, as well as a neomycin resistance cassette (PGK-Neo-pA) for selection of the transfected ES cells. |