| First Author | Plaksin D | Year | 1997 |
| Journal | J Immunol | Volume | 158 |
| Issue | 5 | Pages | 2218-27 |
| PubMed ID | 9036968 | Mgi Jnum | J:38504 |
| Mgi Id | MGI:85887 | Doi | 10.4049/jimmunol.158.5.2218 |
| Citation | Plaksin D, et al. (1997) A three-domain T cell receptor is biologically active and specifically stains cell surface MHC/peptide complexes. J Immunol 158(5):2218-27 |
| abstractText | We have expressed in bacteria a single-chain T cell receptor (scTCR) with specificity for an HIV gp120-derived peptide bound to the murine MHC-I molecule, H-2Dd. This scTCR consists of V alpha covalently linked to the VbetaCbeta domains that was solubilized, refolded, and purified in high yield. Specific binding of the scTCR to MHC/peptide complexes was demonstrated by surface plasmon resonance, with a Kd of 2 to 8 x 10(-6) M. This scTCR specifically inhibited T cell activation, and stained cell surface MHC/peptide complexes as measured by cytofluorimetry. The preservation of binding specificity by such a three-domain scTCR suggests that this structure is sufficient for specific MHC/peptide recognition and that this strategy will be of general use as applied to other TCR. |
