| Primary Identifier | MGI:6438218 | Allele Type | Endonuclease-mediated |
| Gene | Atp1a3 | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Plasmids encoding a signal guide RNA are designed to introduce the D801N point mutation in exon 17. An additional silent nucleotide change was introduced as part of the CRISPR/Cas9 targeting strategy. This change of CTG to CTA (L802L) has no effect on protein sequence. Correctly targeted pups were identified by sequencing and PCR. |
